• Featured Discovery

    Home > Featured Discovery > SJTU Research Team Unraveled Secret of Quality Protein Maize

    SJTU Research Team Unraveled Secret of Quality Protein Maize

    January 08, 2020      Author: School of Agriculture and Biology

    On January 7, 2020, a paper entitled "Long-read Sequencing Reveals Genomic Structural Variations That Underlie Creation of Quality Protein Maize" was published  in online Nature Communications. The research behind the paper was conducted by Wang Wenqin's team from the School of Agriculture and Biology, Shanghai Jiao Tong University together with Wu Yongrui's team from CAS Center for Excellence in Molecular Plant Sciences. Their research has uncovered a puzzle on quality protein maize breeding which has lasted for over half a century.

    Li Changsheng from the School of Agriculture and Biology, SJTU, and Xiang Xiaoli, Postdoctoral fellow from the CAS Center for Excellence in Molecular Plant Sciences, are co-first authors of this paper. Wang Wenqin, School of Agriculture and Biology, SJTU, and Wu Yongrui, CAS Center for Excellence in Molecular Plant Sciences, are the corresponding authors of this paper. The research has received strong support from the Ministry of Science and Technology, the National Foundation of China, and the Chinese Academy of Sciences.

     

    Abstract

    Mutation of o2 doubles maize endosperm lysine content, but it causes an inferior kernel phenotype. Developing quality protein maize (QPM) by introgressing o2 modifiers (Mo2s) into the o2 mutant benefits millions of people in developing countries where maize is a primary protein source. Here, we report genome sequence and annotation of a South African QPM line K0326Y, which is assembled from single-molecule, real-time shotgun sequencing reads collinear with an optical map. We achieve a N50 contig length of 7.7 million bases (Mb) directly from long-read assembly, compared to those of 1.04 Mb for B73 and 1.48 Mb for Mo17. To characterize Mo2s, we map QTLs to chromosomes 1, 6, 7, and 9 using an F2 population derived from crossing K0326Y and W64Ao2. RNA-seq analysis of QPM and o2 endosperms reveals a group of differentially expressed genes that coincide with Mo2 QTLs, suggesting a potential role in vitreous endosperm formation.

    Paper Link: https://www.nature.com/articles/s41467-019-14023-2

     

    Translated by Zhu Fengyan     Reviewed by Wang Bingyu

     
    意甲下注app首页

    美国足球联赛下注

    |

    足彩进球彩玩法

    |

    竞彩足球怎么买才稳赚

    |

    皇冠竞彩足球

    |

    亚盘足彩什么app可以买

    |

    足球比赛规则

    |

    竞彩足球app官方版

    |

    足彩2串1最有效技巧

    |